Laboratory Handbook for the Fractionation of Natural ExtractsSpringer Science & Business Media, 6 Des 2012 - 199 halaman This laboratory manual will be welcomed by all research scientists involved in the extraction, fractionation and isolation of compounds from natural materials, especially those working with plants. The book is clear and concise, and features practical exercises to illustrate the techniques described in every chapter. It will provide an invaluable research reference tool for those scientists investigating the potential benefits of ethnomedicine and the properties of chemicals isolated from natural flora. |
Isi
| 1 | |
Important concepts | 14 |
Methods for extraction and sample cleanup | 22 |
Crude fractionation procedures | 54 |
Chromatographic procedures using columns and liquidliquid | 82 |
Layer chromatography | 94 |
Analysis of crude extracts fractions and isolated compounds | 113 |
General methods | 139 |
Practical exercises in chemical screening and fractionation | 154 |
Appendix A Recipes for chemical test reagents | 184 |
Edisi yang lain - Lihat semua
Laboratory Handbook for the Fractionation of Natural Extracts Peter J. Houghton,Amala Raman Pratinjau tidak tersedia - 1998 |
Laboratory Handbook for the Fractionation of Natural Extracts Peter Houghton,Amala Raman Pratinjau tidak tersedia - 2012 |
Istilah dan frasa umum
absorbance acetone achieved adsorption aglycone alcohol aliquots alkaloids analytical TLC anthraquinones applied aqueous extract bands biological boiling Buchner caffeine cardenolides cellulose Centrifuge chloride chloroform chlorophyll clean-up colour column chromatography components concentration constituents containing detection dichloromethane dilute dissolved distillation eluant eluted ethyl acetate evaporating dish Figure filter filtration Fixed oils flask fluid fractionation glycosides heating hexane HPLC ion-exchange isolated layer less polar methanol method mixture mobile phase molecules natural products non-polar obtained partition phenolic phenolic compounds plant material polar solvents polyphenols polyvinylpolypyrrolidone pores powdered precipitate preparative present pressure procedures proteins R₁ values range removed resin reverse-phase Rotary evaporator sample Sephadex silica gel sodium solubility solution solvent extraction spray reagent stationary phase Step substances sugars sulphuric acid supernatant technique temperature thin-layer TLC plate tube types usually UV light vacuum volatile oils volume water bath zones