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Mr. Charles Stewart: Sporogonia of Funaria hygrometrica, and some new Polyzoa.

Mr. Amos Topping: Various patterns of grouped Polycystina, and some injected preparations mounted in balsam, but preserving the true form as in fluid mounting.

Mr. F. H. Ward: New form of micro-spectroscope; spectrum of didymium, didymium glass, &c.; and sections of the stem of Eucalyptus stained with carmine, logwood, &c.

Mr. Robert G. West: Lissajou's curves on glass slides for the microscope.

KING'S COLLEGE, LONDON, June 5, 1878.

H. J. Slack, Esq., President, in the chair.

The minutes of the preceding meeting were read, and were signed by the President.

A list of the donations received since the last meeting was read by the Secretary, and the thanks of the Society were voted to the respective donors.

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The President said that under the old practice of the Society its meetings took place on the second Wednesday in the month; but when the Monthly Microscopical Journal' was commenced, it was represented to them by the Publisher that this did not give sufficient time to allow of the Proceedings' appearing in the next monthly issue. On this account their night of meeting was altered to the first Wednesday in the month, which had been found to clash with the meetings of the Geological Society and some others, to the great inconvenience of many of the Fellows. Under the new arrangements for the Journal there would be no difficulty in regard to the publication of the Proceedings' if their meetings were held later in the month; and it had therefore been suggested that they should revert to the original plan of meeting on the second instead of on the first Wednesday in the month. Their next meeting would not take place until October, before which time a new list of meetings would be issued, and in which the alteration would appear. It was not quite formal to bring the question before an ordinary meeting, but the Council-with whom it rested to make the change-thought it better to mention it this evening, so that if any Fellow had any objection he might be able to state it. All that the Council wished was to accommodate the greatest number as much as possible.

There being no objection raised, the President formally announced that in future the meetings of the Society would be held on the second Wednesday in each month during the session.

The President said the meeting would be pleased to hear that they were favoured with the presence that evening of Professor Stokes, Sec. R.S., who had come up from Cambridge to read a paper he had prepared on the angular aperture of object-glasses, and which it was considered would entirely dispose of the points formerly in controversy on this question. Before he read it, the Secretary would read a note by Professor Keith, bearing on the same subject.

Mr. Frank Crisp (Secretary) then read the note by Professor Keith,

which was entitled "On the Results of a Computation relating to Tolles' Objective." It was accompanied by photographs of the Professor's computation and of his diagram of the objective, showing the lenses of which it was composed and the path of a ray through it.

Professor Stokes, after disclaiming for his paper all the importance attached to it by the President, proceeded to read it with comments, illustrating it by reference to diagrams, and by drawings upon the black-board.

Mr. Ingpen ventured to make a few remarks on the practical bearing of the paper they had just heard. The theory was evidently correct, and would not be doubted by anyone acquainted with the more advanced optics, but there was some practical difficulty in securing an illuminating pencil which could utilize the increased angle of aperture obtained by the new arrangement. This could not be done by any ordinary condenser, where the light impinged upon the under side of a flat surface, but it was effected by Professor Abbe's immersion illuminator, and others constructed on similar principles. Another difficulty-one for the optician-was to construct such middle and back combinations for the objective as would utilize the large cone of rays entering the front hemisphere. This Professor Abbe had certainly achieved to the extent of 113°, which was a great advance on all previous apertures.

Mr. J. W. Stephenson (Treasurer) said it was true that they were limited to an equivalent angle of 180° in air if they had a plane dry surface beneath the slide; to get the full effect they must, of course, have some medium to connect the condenser with the balsamed object instead of air; Professor Abbe had now devised an immersion condenser having a balsam angle of 138°.

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Mr. Mayall, jun., said that Professor Abbe had not been the first to devise means of illumination far exceeding the limit that obtains when the base of the slide is flat and dry. A lens almost exactly similar to Professor Abbe's was figured by Mr. Wenham in the Quarterly Journal' more than twenty years ago, and the purpose was the same,— to obtain extremely oblique illumination. Mr. Wenham suggested its use with the paraboloid. Then we had the reflex illuminator, that works well up to a moderate limit; the immersion semi-cylinder that permits the rays to fall on the object, when mounted on the slide or in balsam, at an inclination approximating to 90° in glass, and various forms of immersion prisms, among which Dr. Woodward's is particularly practical. He did not understand Mr. Ingpen's difficulty in providing oblique rays of sufficient intensity for practical use, as the difficulty he had found was to regulate the amount of the light, not its obliquity. For example: by blocking out the whole of that part of the illuminating pencil on a balsamed object that corresponds to the air pencil of 180°, and using only rays beyond this inclination, he had found, with objectives having balsam angle of 95° and upwards, the more difficult images were made more appreciable by the eye. It appeared to him it was the excess of angle beyond 82° in glass of the illuminating pencil that enables us to see the more difficult images; and so it is with the aperture of the objective. In the demonstration

given by Professor Stokes the object is supposed to be self-luminous, emitting a pencil of rays of 180° in glass, which pencil was shown to pass by a single refraction, without aberration, from the front lens, and to present to the second lens a pencil in air of only just beyond 81°. Professor Stokes did not say the whole of this pencil can be made available in a practical construction, but he stated that a very considerable portion of it-largely in excess of what is available in a dry lens-could be so used. After this demonstration the question of the possibility of immersion lenses having apertures exceeding the maximum possible in dry lenses would be settled from the theoretical point of view. His opinion on the validity of Professor Keith's computation of the aperture of Tolles' -Mr. Crisp's lens-must also be conclusive. It remained to endeavour to support the computation by actually measuring the aperture of the lens before the meeting-which he proposed doing, not of course expecting any general agreement at this stage of the discussion as to the least objectionable way of measuring apertures, especially with this individual lens. He would be content to show the measurement by Professor Abbe's apertometer, the results obtained with which he had found to correspond with those obtained by a modification of Professor Robinson's method which was submitted to Professor Stokes, and for the accuracy of which he had his authority. A method was specially commended by Mr. Charles Brooke in one of his Presidential addresses, viz.: to measure the working diameter of the front lens, which is taken as the base line of an isosceles triangle; taking the exact focal distance as the perpendicular, the triangle would represent the angle of aperture. The objection to this method in practice, was that when it was tried with the lens, the data furnished were so various and contradictory that no reliance could be placed on the results: in one case the focal distance being given as 0.13, then 018; in another 025,-the working diameter being given first as 043, then as ⚫033.

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Professor Stokes said that of course it would be understood that, as stated by Mr. Mayall, he confined himself to the consideration of a point as if it were self-luminous, and contemplated that the object was illuminated by immersion; if they let in light to the plane surface in air they would be limited to twice the critical angle.

The President announced that they had received a letter from Professor Abbe, thanking the Society for the honour done to him by his election as an Honorary Fellow of the Society.

Mr. Charles Stewart (Secretary) read a paper by the Rev. W. H. Dallinger, "On the Measurement of the Diameter of the Flagella of Bacterium termo, a contribution to the question of the ultimate limits of vision with our present lenses."

The President said that what must have struck everyone who had listened to this paper was the extreme smallness of the objects measured, for if Mr. Dallinger was correct (and there was no reason for doubting his correctness), then it was clear that objects of very much greater minuteness could be rendered visible than had usually been considered possible. These objects were about the smallest which the microscope would show, and yet it appeared that they

probably stood higher in the scale of organisms than some other objects which were generally considered to be higher. He thought that these investigations of Mr. Dallinger would tend to raise such objects to a much higher rank than that previously assigned to them.

The President said they had two other papers before them; one of these was from Mr. F. A. Bedwell, "On the Framework of the Mastax of Melicerta ringens and Conochilus," and the other was a translation by Mr. Kitton of a paper by M. Paul Petit, " On some New Genera and Species of Diatomaceæ." The latter paper was, of course, unsuitable for reading in extenso, and would be taken as read. The paper by Mr. Bedwell was of considerable interest, and went into the minute structure of the organ at some length; but in his opinion, apart from the illustrations (which would be handed round), it would scarcely be possible to do full justice to the author or his subject in the time left at their disposal, and it would therefore now be taken as read, and would appear in the Journal together with the illustrations in September. He would also call attention to the extremely good slides of the mastax of Melicerta and Conochilus made by Lord S. G. Osborne, which had been sent up by Mr. Bedwell for examination by the meeting.

Mr. J. W. Stephenson (Treasurer) read a "Note on the effect produced on P. angulatum and other Tests by excluding the central dioptric beam of light," which he explained was accomplished by placing a central stop at the back of the objective, so that the diffraction spectra alone formed the image.

Mr. Frank Crisp (Secretary) said that, in view of the four months' vacation now commencing, he should like to call the attention of the Fellows to the experiments on diatoms immersed in a solution of indigo or other coloured liquid, a note of which had appeared in the last number of the Journal (vol. i. p. 79), and the important conclusions that those experiments pointed to, particularly in regard to the motions of diatoms. The experiments were well worthy of being repeated and confirmed, and if any Fellow during the vacation would be kind enough to do so, he would, no doubt, be able to make a very interesting communication to the Society when they met again.

The President suggested that Professor Stokes should take up the question of the limits of visibility, which in his hands would doubtless receive some important advancement towards a conclusion. He also mentioned that some specimens of drawings reproduced by the autographic process (which was exhibited at their recent scientific evening, by Mr. Pumphrey, of Birmingham) had been sent to the Society for distribution.

At the conclusion of the meeting Mr. Stephenson exhibited P. angulatum under the conditions described in his "Note," and with the new oil-immersion lens, using a deep astronomical piece by Dollond, The diatom appeared of a brilliant blue on a perfectly black ground, and the definition left nothing to be desired.

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Mr. Mayall, jun., also demonstrated the aperture of Tolles' immersion lens by Professor Abbe's apertometer. He first exhibited the apertometer with Zeiss's new oil-immersion lens, recording

a balsam angle greater than 106°; then he showed the aperture of a high-angled dry lens to be within double the critical angle, i. e. less than 82° in glass; then he showed the aperture of Tolles' immersion to be in excess of the limit for dry lenses, i. e. greater than 82° in glass. The demonstration was conducted in the presence of Professor Stokes, Mr. W. G. Lettsom, Mr. J. H. Dallmeyer, Mr. F. Crisp, and others interested in the subject.

The meeting was then adjourned to the 9th October, and the President reminded the Fellows that the library and reading room would as usual be closed during the month of August.

Donations to the Library, &c., since April 3, 1878:

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Papers and Proceedings of the Royal Society of Tasmania, 1876
Transactions of the Watford Natural History Society. Part 9
Journal of the Linnean Society: Zoology.

No. 74

Report and Abstract of Proceedings of the Croydon Microscopical

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S. S. Wigg, Esq.

The following gentlemen were elected Fellows of the Society :Dr. James Edmunds; Major Richard O'Hara.

WALTER W. REEVES,

Assist.-Secretary.

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