Molecular Cloning: A Laboratory Manual, Volume 1

Sampul Depan
CSHL Press, 2001 - 2344 halaman

The first two editions of this manual have been mainstays of molecular biology for nearly twenty years, with an unrivalled reputation for reliability, accuracy, and clarity.
In this new edition, authors Joseph Sambrook and David Russell have completely updated the book, revising every protocol and adding a mass of new material, to broaden its scope and maintain its unbeatable value for studies in genetics, molecular cell biology, developmental biology, microbiology, neuroscience, and immunology.
Handsomely redesigned and presented in new bindings of proven durability, this three–volume work is essential for everyone using today’s biomolecular techniques.
The opening chapters describe essential techniques, some well–established, some new, that are used every day in the best laboratories for isolating, analyzing and cloning DNA molecules, both large and small.
These are followed by chapters on cDNA cloning and exon trapping, amplification of DNA, generation and use of nucleic acid probes, mutagenesis, and DNA sequencing.
The concluding chapters deal with methods to screen expression libraries, express cloned genes in both prokaryotes and eukaryotic cells, analyze transcripts and proteins, and detect protein–protein interactions.
The Appendix is a compendium of reagents, vectors, media, technical suppliers, kits, electronic resources and other essential information.
As in earlier editions, this is the only manual that explains how to achieve success in cloning and provides a wealth of information about why techniques work, how they were first developed, and how they have evolved.

 

Isi

Chapter 12
12
Chapter 9
11
1
13
In Vitro Amplification of DNA by the Polymerase 8 1
8
Preparation of Radiolabeled DNA and RNA Probes 9 1
9
INTRODUCTION
3
Chapter 4
4
PROTOCOLS
16

INTRODUCTION
16-13
Calciumphosphatemediated Transfection of Cells with Highmolecularweight 16 21
16-21
DNA Transfection Using Polybrene 16 43
16-43
Chapter 1
17-1
Chapter 17
17-3
Gel Electrophoresis of DNA and Pulsedfield Agarose 5 1
17-5
Transcriptional Runon Assays 17 23
17-23
INFORMATION PANELS
17-75
INFORMATION PANELS
17-91
INFORMATION PANELS
17-99
Chapter 18
18-3
Extraction Purification and Analysis of mRNA from 7 1
18-7
Chapter 10
18-10
Chapter 14
18-14
INFORMATION PANELS
18-82
INFORMATION PANELS
18-107
INFORMATION PANELS
18-115
Appendices
18-137
Chapter 13
13
1
30
INFORMATION PANELS
6
Chapter 8
8
Working with Highcapacity Vectors 4 1
4
INTRODUCTION
10
DNA Sequencing 12 1
12
Working with Synthetic Oligonucleotide Probes 10 1
10
INTRODUCTION
R-20
1
i
PREFACE XV
xv
Chapter 2
2
Screening Expression Libraries 14 1
14
1
56
Chapter 4
59
7
75
11
84
INFORMATION PANELS
109
Amplifiers
144
AnalogtoDigital Converters
155
References
165
CALIBRATION AND RESPONSE 93
173
577
183
59599
203
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