Laboratory Manual In Microbiology

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New Age International, 1 Jan 2007 - 152 halaman
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This Manual Is Intended To The Undergraduate And Post-Graduate Students In Microbiology As Well As Botany And Zoology In Which Microbiology Is Being Taught As Ancillary Subject. This Manual Explains Exercises In Simple Terms With Sufficient Background And Principle Of The Experiments. Illustrations Are Provided Along With The Protocols For Effective Understanding The Experiments. This Manual Deals With The Experiments In Basic Microbiology, Microbial Physiology Metabolism, Soil, Agricultural, Water And Medical Microbiology. It Is Expected That Beginners And Graduate Students In Microbiology Will Be Benefited From This Manual.
 

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GENERAL LABORATORY RULES MICROSCOPY
1
PREPARATlON OF GLASSWARES MEDIA STERILIZATION
6
Preparation of culture media for bacteria
9
Distribution of microorganisms in our environment
13
Pure culture of bacteria
16
Aseptic transfer of pure cultures
20
Measurement of the size of bacterial cell
22
Motility of bacterial cell
26
Bacterial survival against UV irradiation and mutagenesis
78
lsolation of petite mutants of yeast
82
Transformation
85
Conjugation in bacteria
87
Microbiological examination of water
91
Bacteriological testing of milk
98
Microbial population in soil
101
lsolation of nitrogen fixing organisms
103

Staining of microorganisms
28
Measurement of cell number in a culture
39
Measurement of growth of microorganisms
47
Fermentation of simple carbohydrates
53
Extracellular enzymes of bacteria
56
ldentification of products of metabolic pathways
59
lsolation of bacteriophages
65
Preparation of bacteriophage stock
66
Titration of bacteriophages
67
One step growth of bacteriophages
68
Microbial antibiosis
73
Antibiotic assay
75
Observation of Mycorrhizal fungi
105
Alcohol fermentation by yeast
108
lsolation of bacterial pigments
111
Evaluation of antimicrobial chemical agents
113
lsolation and characterization of hemolytic streptococci
116
lsolation and identification of staphylococci
119
lsolation and characterization of intestinal pathogens
121
STORAGE OF CULTURES
123
MEDIA
125
STAlNS AND REAGENTS
135
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