Laboratory Manual In MicrobiologyNew Age International, 2007 - 152 halaman This Manual Is Intended To The Undergraduate And Post-Graduate Students In Microbiology As Well As Botany And Zoology In Which Microbiology Is Being Taught As Ancillary Subject. This Manual Explains Exercises In Simple Terms With Sufficient Background And Principle Of The Experiments. Illustrations Are Provided Along With The Protocols For Effective Understanding The Experiments. This Manual Deals With The Experiments In Basic Microbiology, Microbial Physiology Metabolism, Soil, Agricultural, Water And Medical Microbiology. It Is Expected That Beginners And Graduate Students In Microbiology Will Be Benefited From This Manual. |
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GENERAL LABORATORY RULES MICROSCOPY | 1 |
PREPARATION OF GLASSWARES MEDIA STERILIZATION | 4 |
Preparation of culture media for bacteria | 4 |
Distribution of microorganisms in our environment | 13 |
Pure culture of bacteria | 16 |
Aseptic transfer of pure cultures | 20 |
Measurement of the size of bacterial cell | 22 |
Motility of bacterial cell 288 | 26 |
Bacterial survival against UV irradiation and mutagenesis | 78 |
Isolation of petite mutants of yeast | 82 |
Transformation | 85 |
Conjugation in bacteria | 87 |
Transduction | 89 |
Microbiological examination of water | 91 |
Bacteriological testing of milk | 98 |
Microbial population in soil | 101 |
Staining of microorganisms | 28 |
Measurement of cell number in a culture | 39 |
Measurement of growth of microorganisms | 47 |
Fermentation of simple carbohydrates | 53 |
Extracellular enzymes of bacteria | 56 |
Identification of products of metabolic pathways | 59 |
Isolation of bacteriophages | 65 |
Preparation of bacteriophage stock | 66 |
Titration of bacteriophages | 67 |
One step growth of bacteriophages | 68 |
Microbial antibiosis | 73 |
Antibiotic assay | 75 |
Isolation of nitrogen fixing organisms | 103 |
Observation of Mycorrhizal fungi | 105 |
Alcohol fermentation by yeast | 108 |
Isolation of bacterial pigments | 111 |
Evaluation of antimicrobial chemical agents | 113 |
Isolation and characterization of hemolytic streptococci | 116 |
Isolation and identification of staphylococci | 119 |
Isolation and characterization of intestinal pathogens | 121 |
APPENDIX | 123 |
MEDIA | 125 |
STAINS AND REAGENTS | 135 |
Istilah dan frasa umum
acid agar medium Agar per litre agar plate Air dry alcohol aminoacid antibiotic autoclave Background bacterial cells bacterial culture bacteriophages Bromothymol Blue broth carbohydrate cell number Citrate coliforms colonies colour cool cotton Crystal violet disinfectant Distilled water EXERCISE eye piece fermentation flame flask Genetic Glucose glycerol gm gm Gram positive Gram Staining growth haemocytometer Incubate at 37°C Incubate the plates inoculation loop isolated K₂HPO4 labelled Lactose lactose broth LB agar plate lens magnification Materials required method Methylene Blue Microbial Microbiology microorganisms microscope milk nitrogen number of cells nutrient agar nutrient agar plate nutrient broth Observe ocular micrometer oil immersion objective overnight Peptone petite mutants petriplates phage Phenol Pipettes plaque plasmid plug Prepare Procedure produce Pseudomonas reagent sample Serially dilute smear Sodium soft agar soil solution SSLB stage micrometer stain Staphylococcus sterile streak Streptococci subtilis test tubes transfer water bath